The sample was collected in a wooded area where a small river was flowing through and it was a mile away from a farm. For inoculation results, the inoculation samples turned yellow in minimal media subcultures containining paraoxon (Px) which indicates production of P-nitrophenol due to OP hydrolysis. The color was brighter in CSM mediumcompared that in SSM medium whichsuggestthat the bacteria prefer carbon as their food source. As for subcutlures containig methyl parathion (MP), they showed no colors after a series of inoculation. For screeningresult, the individual colonies and halos were visible onthe CMM plate. In conclusion, the soil sample contained bacteria capable of expressing organophosphorus (OP) hydrolase and breaking down OP compound.