The sample was collected near Memorial Park Conservancy. This place likely to have pesticide use or activity, because it is expected to discover organophosphate enzymes where pesticides are used, the collection of the sample was performed near an area where pesticides are ubiquitous. Following several inoculations, screening was carried out on the CMM plate to detect OP hydrolysis. The bacterial growth essentially indicated resistance to the OP compounds, whereas the “halo effect” was the evidence for OP hydrolysis. Moreover, CSM subcultures indicated a more intense color, as compared to the SSM subcultures. The findings of the Screening results indicated countless colonies with the “halo effect” on the CMM subculture. In summation, this experiment indicated a positive result, in that the collected soil possessed microorganisms that were able to express the organophosphorus hydrolase enzyme and organophosphate degradation.