An 12 inches hole was dig and soil was obtain. The soil sample was first inoculated in an enriched media and incubate at 37 degree Celsius. The soil containing media was then pipettes in minimal medical containing paraoxon and methyl parathion. Again was incubate for 7 day at 37 degree Celsius. This process was repeated until week 5. Then plated onto minimal agar with pesticide to observe for OP hydrolysis.