Pesticide Used: Bugbgon Ortho Max insect killer for lawns Site description: home front lawn Negative staining: showed a bacillus shaped cell about 4.8 micrometers. Agar deep showed that the bacteria is a facultative anaerobe (growth on top of deep and slightly below). Gram stain showed gram negative (pink) bacteria that was bacillus shaped. Each staining showed the bacterial cells in a singular collection. The cells are diderms since they are gram negative. Gram staining resulted in cells that were 2 micrometers which indicates that my cell also contained a capsule since it was smaller than the negative stain. Catalase test came back positive because the sample bubbled on contact. Hanging drop motility test showed that the cells only exhibited Brownian motion because they vibrated in place. This indicates that there are no flagella. Oxidase test was also positive and turned purple within the 30 seconds. Acid from glucose test came back positive because the broth turned a slight pink color from the dark red. Nitrates reduction test came back negative because there was no color change that happened in the broth. Using the key from the ABIS database I was able to conclude that the genus of the bacteria is Ornithobacterium or Cardiobacterium. Electrophoresis showed that my sample is about 1,300 bp. After doing a BLAST search the closest bacterial species Achromobacter insuavis Percent identity: 99.37% %Query cover: 100% Accession number: MT534120.1.
2021 Spring - SUU - Thu - McKell Ferry
Description
Data for Soil Sample and Researcher
Location of Soil Sample
A home front lawn, 728 S Ridge Rd, Cedar City, UT 84720, United States, 37.665, -113.084
Collection Date of Soil Sample
Colonies of Paraoxon
0
Colonies of Methyl Parathion
0
Researcher
McKell Ferry
Organization
Southern Utah University
Lab Day
Thursday
Course Term
Spring
Year of Course
2021
Extended Analysis Results
16S rDNA File
Most Likely Isolate
Achromobacter insuavis